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Coccidioides Complement Fixation (Serum)
Test Code18393
CPT Codes
86635<br><strong>This code is not available for New York, Maryland and Rhode Island patient testing.</strong><br><strong>Restricted Use. This is a TSO EXCEPTION for clients 4074, 50515, 2619, 55514, 55518, 56250, 56182, 57340, 57567, 57614, 58340, 59104, Sutter and Kaiser ONLY.</strong>
Includes
If Coccidioides Complement Fixation is Anticomplementary or Equivocal, then Quantitative Immunodiffusion will be added at an additional charge (CPT Code(s): 86317).
Preferred Specimen
2 mL serum
Minimum Volume
0.5 mL
Instructions
Allow serum samples to clot at room temperature. Please separate serum from cells prior to sending. Even when using serum separator tubes, serum should be transferred into another tube as the chemical composition of the serum can change and potentially affect results. For CSF and other body fluids, a sterile container without anticoagulants or other additives is appropriate.
If minimum volume (0.5 mL) sent, then the qualitative immunodiffusion screen will be performed on an unconcentrated specimen which may decrease sensitivity. Quantitative immunodiffusion will be performed to obtain a serology titer.
Interferences: Gross hemolysis or icterus may affect visualization of reactions by immunodiffusion Gross lipemia may cause interferences with complement fixation
If minimum volume (0.5 mL) sent, then the qualitative immunodiffusion screen will be performed on an unconcentrated specimen which may decrease sensitivity. Quantitative immunodiffusion will be performed to obtain a serology titer.
Interferences: Gross hemolysis or icterus may affect visualization of reactions by immunodiffusion Gross lipemia may cause interferences with complement fixation
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: 60 days
Refrigerated: 7 days
Frozen: 60 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Plasma • Bronchial alveolar lavage fluid • Urine • Grossly purulent fluids • Wound drainage • Swabs • Sputum
Methodology
Complement Fixation (CF)
Setup Schedule
Set up: Mon-Fri; Report available: 7-10 days
Clinical Significance
Recommended for follow up with known positive and a qualitative immunodiffusion result is not required on the report. Quantitative (IgG) detection of antibodies specific to Coccidioides. In the event of insufficient volume or equivocal or anticomplementary results by complement fixation methods, a quantitative immunodiffusion will be performed to determine a titer. A qualitative immunodiffusion test is performed internally for accurate complement fixation serial dilutions and quality control purposes.
If the CF test is positive, residual specimen is frozen by us for later simultaneous testing with a new specimen to control for variability in the CF test (PRVEII). Residual specimen is saved for approximately 4-6 months.
If the CF test is positive, residual specimen is frozen by us for later simultaneous testing with a new specimen to control for variability in the CF test (PRVEII). Residual specimen is saved for approximately 4-6 months.
