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Helicobacter pylori Culture
Test Code16597
CPT Codes
87205, 87081
Preferred Specimen
3 mm gastric/antral biopsy or duodenal biopsy collected in brucella or trypticase soy broth (TSB) with 10-20% glycerol
Other Acceptable Specimens
Gastric/antral or duodenal brushings collected in Brucella broth or trypticase soy broth (TSB) with 10-20% glycerol or
Gastric/antral or duodenal biopsy or brushings collected in Brucella or trypticase soy broth (TSB) without glycerol, or non-bacteriostatic saline
Transport Temperature
Brucella Broth or equivalent with glycerol: Frozen -70°C (dry ice)
Broth with or without glycerol, or sterile non-bacteriostatic saline: Refrigerated (cold packs)
Broth with or without glycerol, or sterile non-bacteriostatic saline: Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen -20° C: Unacceptable
Frozen -70° C: 5 days (glycerol broth only)
Refrigerated: 48 hours
Frozen -20° C: Unacceptable
Frozen -70° C: 5 days (glycerol broth only)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Transport swab with or without gel • Specimens frozen at -20°C • Stool
Methodology
Culture
Setup Schedule
Set up: Daily; Report available: 8-10 days
Clinical Significance
Helicobacter pylori has been associated with peptic ulcer disease and cancers of the gastrointestinal tract.
