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FISH, Myeloid Neoplasms, MECOM (EVI1), 3q26.2
Test Code91391
CPT Codes
88271 (x3), 88275
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Other Acceptable Specimens
Bone marrow or whole blood collected in: Sodium heparin (royal blue-top) tube or Sodium heparin lead-free (tan-top) tube
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report Available: 5-7 days
Clinical Significance
Disruption of the human entropic virus integration site (EVI1 or MECOM) gene located at chromosome band 3q26.2 is associated with myeloid neoplasms and is indicative of a poor prognosis. EVI1 rearrangements are especially frequent in myeloid blast crisis that evolves through tyrosine kinase inhibitors. It has been associated with dysplasia of megakaryocytes and alters differentiation of other myeloid cells. Associated rearrangements include inv(3)(q21q26), t(3;3)q21;q26), t(3;21)(q26;q22) and t(3;12)(q26;p13).