| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Electron Microscopy
Test Code14249
CPT Codes
88348
Preferred Specimen
Tissue in buffered glutaraldehyde (2.5%-3%).
Collection Instructions:
__** This test is not available for New York patient
__testing. **
__
__Specimen: Kidney, Muscle, Nerve, Cilia (Brushing and
__Biopsies). Immediately after removal of tissue, the
__specimen should be immersed in fixative. For samples other
__than listed, please contact the lab at 972-966-7207
__
__1. Please provide patient information including
__ Patient information (name, age, etc.)
__ Patient history or pathologist comments
__ Specimen type
__ Reason for testing
__ Type of fixative
__ Time and duration of fixation
__ Physician contact information
__2. Indicate the patients first and last name and include a
__second unique identifier on each of the vials. The name on
__the vials must match the paperwork being sent with the
__specimens. The specimen source and fixative used must be
__clearly marked on each vial. 3. For needle core biopsies,
__place directly into fixative. Avoid crushing tissue when
__handling. 4. For Renal Biopsies, specimen should be
__examined for the presence of glomeruli prior to submission
__to med fusion. Use a sharp razor blade or scalpel to
__remove at least 1-mm cubes (2-3 glomeruli) from the ends
__of cores for EM. Larger samples should be sectioned into
__1x1x1 mm strips before fixing. 5. For paraffin embedded
__tissue, the area of interest should be marked clearly on
__the microscopic slide corresponding to the block submitted
__for processing. The paraffin block and the slide should
__be labeled with the record number and patient name. 6. For
__Ciliary brushing, immediately immerse the brush in
__fixative.
__
__The results of the ultrastructural evaluation are images
__that will be made available to physicians via the med
__fusion Client Collaboration Portal. Each site/physician
__will be given a unique address and log in credentials to
__access their images. Or the med fusion EM lab provides
__hard copies at the clients request.
__
__Note: paraffin-embedded samples not accepted for Cilia
__
__Do Not Freeze, Do Not Reject
__
__
Collection Instructions:
__** This test is not available for New York patient
__testing. **
__
__Specimen: Kidney, Muscle, Nerve, Cilia (Brushing and
__Biopsies). Immediately after removal of tissue, the
__specimen should be immersed in fixative. For samples other
__than listed, please contact the lab at 972-966-7207
__
__1. Please provide patient information including
__ Patient information (name, age, etc.)
__ Patient history or pathologist comments
__ Specimen type
__ Reason for testing
__ Type of fixative
__ Time and duration of fixation
__ Physician contact information
__2. Indicate the patients first and last name and include a
__second unique identifier on each of the vials. The name on
__the vials must match the paperwork being sent with the
__specimens. The specimen source and fixative used must be
__clearly marked on each vial. 3. For needle core biopsies,
__place directly into fixative. Avoid crushing tissue when
__handling. 4. For Renal Biopsies, specimen should be
__examined for the presence of glomeruli prior to submission
__to med fusion. Use a sharp razor blade or scalpel to
__remove at least 1-mm cubes (2-3 glomeruli) from the ends
__of cores for EM. Larger samples should be sectioned into
__1x1x1 mm strips before fixing. 5. For paraffin embedded
__tissue, the area of interest should be marked clearly on
__the microscopic slide corresponding to the block submitted
__for processing. The paraffin block and the slide should
__be labeled with the record number and patient name. 6. For
__Ciliary brushing, immediately immerse the brush in
__fixative.
__
__The results of the ultrastructural evaluation are images
__that will be made available to physicians via the med
__fusion Client Collaboration Portal. Each site/physician
__will be given a unique address and log in credentials to
__access their images. Or the med fusion EM lab provides
__hard copies at the clients request.
__
__Note: paraffin-embedded samples not accepted for Cilia
__
__Do Not Freeze, Do Not Reject
__
__
Other Acceptable Specimens
1 10% neutral buffered formalin-fixed tissue or
Paraffin-embedded tissue
Paraffin-embedded tissue
Transport Container
Tissue in buffered glutaraldehyde (2.5%-3%): Sterile
leak-proof container
1 10% neutral buffered formalin-fixed tissue or
Paraffin-embedded tissue
__Sterile leak-proof container
__Paraffin-embedded blocks
leak-proof container
1 10% neutral buffered formalin-fixed tissue or
Paraffin-embedded tissue
__Sterile leak-proof container
__Paraffin-embedded blocks
Transport Temperature
Tissue in buffered glutaraldehyde (2.5%-3%): Room
_temperature preferred; Refrigerated acceptable; Frozen
_unacceptable
1 10% neutral buffered formalin-fixed tissue and
_Paraffin-embedded tissue: Refrigerated preferred; Room
_temperature acceptable; Frozen unacceptable
_temperature preferred; Refrigerated acceptable; Frozen
_unacceptable
1 10% neutral buffered formalin-fixed tissue and
_Paraffin-embedded tissue: Refrigerated preferred; Room
_temperature acceptable; Frozen unacceptable
Specimen Stability
Tissue in buffered glutaraldehyde (2.5%-3%)
__Room temperature: 1 Year
__Refrigerated: 1 Year
__Frozen: Unacceptable
1 10% neutral buffered formalin-fixed tissue or
Paraffin-embedded tissue
__Room temperature: Acceptable
__Refrigerated: Preferred
__Frozen: Unacceptable
__Room temperature: 1 Year
__Refrigerated: 1 Year
__Frozen: Unacceptable
1 10% neutral buffered formalin-fixed tissue or
Paraffin-embedded tissue
__Room temperature: Acceptable
__Refrigerated: Preferred
__Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Alternate Fixatives or improper fixation or, improper
preparation, No fixative on specimen, Specimen not present
in container or, immersed in fixative, Tissue from cases of
suspected transmissible, spongiform encephalopathies (TSE),
including Creutzfeldt-Jakob disease(CJD)
preparation, No fixative on specimen, Specimen not present
in container or, immersed in fixative, Tissue from cases of
suspected transmissible, spongiform encephalopathies (TSE),
including Creutzfeldt-Jakob disease(CJD)
Methodology
Microscopy
Setup Schedule
Sets up 6 days a week.
Report Available
Reports in 3 to 7 days.
Clinical Significance
Electron microscopy uses specialized microscopes with a beam of electrons as the source of illumination. Electron optics are analogous to glass lenses of an optical light microscope. Wavelengths of an electron can be up to 100,000 times shorter than that of visible light. Therefore, electron microscopes provide higher resolutions of about 0.1 nm, compared to about 200 nm with light microscopy.
