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KIT (D816V) Mutation by ddPCR, Quantitative
Test Code12788
CPT Codes
81273
Preferred Specimen
5 mL whole blood or 3 mL bone marrow collected in EDTA (lavender-top) tube
Minimum Volume
1 mL whole blood • 1 mL bone marrow
Other Acceptable Specimens
Sodium heparin (green-top) tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Plasma • Serum • FFPE tissue blocks/slides or fresh or frozen tissue • Specimens collected in anticoagulants other than EDTA or sodium heparin • Clotted specimens
Methodology
Droplet Digital PCR (ddPCR)
Setup Schedule
Set up: Varies; Report available: 2-9 days
Clinical Significance
Aids in the diagnosis of mastocytosis. Provides prognostic and predictive information for tyrosine kinase inhibitor (TKI) therapy planning.
