A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
FISH, MDM2 12q15/CEP 12
Test Code92216
CPT Codes
88271 (x2), 88274
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Minimum Volume
3 slides unstained 5 micron slides on charged (+) slides
Other Acceptable Specimens
5 slides unstained 5 micron slides on charged (+) slides
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5-7 days
Clinical Significance
The differential morphological diagnosis of soft tissue tumors is often complex, including benign versus malignant and sarcoma versus other neoplasms. The dual color MDM2/CEP12 probe set is used to evaluate cultured cells and interphase cells from FFPE for relative gain/ amplification of the MDM2 (transformed mouse 3T3 cell double minute, p53 binding protein) gene at band 12q15. MDM2 amplification may be found in a number of cancers, such as osteosarcomas, esophageal carcinomas, and other soft tissue derived tumors. MDM2 is implicated in a number of cancers due to its relationship with the p53 tumor suppressor gene. As a ubiquitin-protein ligase that promotes p53 degradation, overexpression of the MDM2 gene can contribute to the oncogenesis. Liposarcomas often have complex karyotypes, including large ring chromosomes and giant chromosomes. Molecular characterization using FISH probes is especially useful in differentiating between benign lipomas which are disomic for MDM2 and well differentiated liposarcoma and dedifferentiated liposarcoma which exhibit MDM2 gain/amplification.