|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
HIV-1 BY QUANT NAAT WITH REFLEX TO HIV-1 DRUG RESISTANCE BY NGS
Test CodeLAB3000870
Includes
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is ≥400 copies/mL, then HIV-1 Genotype will be performed at an additional charge (CPT code(s): 87900, 87901).
Preferred Specimen
2 mL plasma collected in each of two EDTA (lavender-top) tubes
Minimum Volume
1 mL (x2)
Other Acceptable Specimens
Plasma collected in each of two PPT (white-top) tubes
Instructions
Samples submitted in primary tube will be rejected. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 42 days
Refrigerated: 6 days
Frozen: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia • Samples received in primary collection tube
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Mon-sat
Report Available
2-5 days
Limitations
An infection is often composed of a variety of strains of the virus. The test may identify only the predominant type of virus thus leaving the minority viruses to thrive once the predominant type is treated. Lack of resistance may not confer susceptibilities.
Clinical Significance
Measurement of HIV-1 plasma levels (viral load) provides a direct assessment of viremia and should be used in conjunction with CD4+ T-cell counts. HIV-1 RNA, quantitation is useful in patients to assess prognosis, monitor progression of HIV-1 infection, determine when to initiate therapy, and monitor the effectiveness of antiretroviral therapy.
