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von Willebrand Factor Collagen Binding Assay
Test Code10924
Preferred Specimen
1 mL frozen plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 24 hours
Frozen: 21 days
Refrigerated: 24 hours
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Icteric specimen
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
FDA Status
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.
Setup Schedule
Night Sets up 2 days a week.
Report Available
Reports in 2 days.
Clinical Significance
The Collagen Binding Activity is a surrogate assay for the measurement of von Willebrand Factor (VWF) mediated Platelet adhesion. Decreased activity (collagen bound to the A3 domain of the VWF protein) relative to the VWF antigen level, is observed with qualitative defects of VWF (type 2 disorders). The CBA assay may assist in the discrimination of type 2 disorders. In addition, there have been reports of isolated defects of CBA as a cause for a variant form of von Willebrand disease.