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HLA-B27 Antigen
Test Code528
Preferred Specimen
5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
EDTA (lavender-top) tube • ACD-A or ACD-B (yellow-top) tube
Transport Container
Sodium heparin (green-top) tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen
Methodology
Flow Cytometry (FC)
FDA Status
This test code is for non-New York patient testing. For New York patient testing, use test code 93099.
Setup Schedule
1st shift; 2nd shift Mon-sun cutoff: 12: 00 PM
Report Available
3 days
Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.
