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Cryoglobulin Screen w/Reflex Cryoglob Profile
Test Code*
Preferred Specimen
10 ml serum Red top tube (no gel)
Minimum Volume
3.5 mL serum (NO GEL)
Instructions
Collect 20 mL of fasting whole blood specimen in a red-top tube (no gel). Avoid hemolysis.
-Allow serum sample to clot for one (1) hour in a 37°C water bath, oven, incubator, or heat block.
-After clotting, centrifuge the specimen for 10 minutes at 3000 rpm utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C; or
2) In a bench-top centrifuge placed in a "warm room" at 37°C; or
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 2000 rpm.
-After centrifuging, decant serum into a screw-capped vial
-Transport at room temperature.
Note: No other tests can be added to the tube; cryocrit and cryoglobulin evaluation only
-Allow serum sample to clot for one (1) hour in a 37°C water bath, oven, incubator, or heat block.
-After clotting, centrifuge the specimen for 10 minutes at 3000 rpm utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C; or
2) In a bench-top centrifuge placed in a "warm room" at 37°C; or
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 2000 rpm.
-After centrifuging, decant serum into a screw-capped vial
-Transport at room temperature.
Note: No other tests can be added to the tube; cryocrit and cryoglobulin evaluation only
Transport Temperature
Room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Refrigerated or frozen specimen
Refrigerated or frozen specimen
Clinical Significance
Send Outs
Performing Laboratory
Quest San Juan Capistrano