BK and JC Virus DNA, Qualitative, Real-Time PCR

Test Code

JCVPCRQT

Quest Code

11068

CPT Codes
87798 (x2)

Preferred Specimen

0.7 mL random clean-catch urine collected in a sterile, plastic, leak-proof container or
0.7 mL plasma collected in an EDTA (lavender-top), ACD (yellow-top), or PPT (white-top) tube

Minimum Volume

0.3 mL

Other Acceptable Specimens

Serum • CSF collected in a sterile, plastic, leak-proof container

Instructions

Urine and CSF: Collect in a sterile, plastic, leak-proof container. Freeze and ship frozen.

Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant. Store collected whole blood at roomtemperature and separate plasma from cells within 2 hours
of collection. Transfer plasma to sterile, plastic, screw-cap, aliquot tubes.

Serum: Collect blood in sterile tubes with no anticoagulants (serum separator tubes) are recommended. Allow blood to clot at room temperature and separate serum from cells within 1 hour of collection. Transfer serum to sterile, plastic, screw-cap aliquot tubes. Freeze and ship frozen.

Transport Temperature

Frozen

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Plasma samples collected with anticoagulants other than EDTA and ACD • Whole blood

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 1 day

Reference Range

Not Detected

Clinical Significance

This test is used to determine the presence of BK and JC Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, rather than the host's reaction to the virus.

Performing Laboratory

Quest Diagnostics Nichols Institute

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.