Gram Stain Smear Routine

Use this test when normal culture does not include a Gram stain. Gram stains are automatically included in the following routine cultures:  body fluid, bronchial washing, burn, sputum, tissue, and wound (aerobic).

Test Code

CPT Codes

Preferred Specimen
Specimen on swab or in sterile container.

Specimen source is required for processing.
  • Collect as for specific bacterial culture.
  • Place specimen in a screw-capped sterile container, a swab transport system or slide prepared with material in a slide holder.
  • Label container (not the lid) or slide with two patient identifiers (full name, DOB or MR), specimen source, and date and time of collection.
  • Maintain sterility and forward promptly

Transport Temperature
See culture requirements

Specimen Stability
Specimen Type Temperature Time
Sterile Container Refrigerate 24 hours
Swab Ambient 48 hours

Gram Stain

Setup Schedule
Monday through Sunday; Continuously

Report Available
Same day

Reference Range
Sterile body sites: no organisms observed.
Critical value (automatic call-back): STAT requests and any positive on spinal fluid or sterile body fluid.

Performing Laboratory
McKee Medical Center - Microbiology/Molecular Laboratory

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.