| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
HIV-2 DNA/RNA, Qualitative, Real-Time PCR
Test CodeHIV-2QLPCR
Quest Code
34977
CPT Codes
87538
Preferred Specimen
1 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
0.4 mL
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Heparinized whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1-2 days
Reference Range
Not detected
Clinical Significance
Infection with Human Immunodeficiency Virus type 2 (HIV-2) is currently diagnosed by the presence of antibodies to HIV-2, the detection of specific HIV-2 antigens or the ability to culture HIV-2 from blood, fluid or tissue of infected persons. The Polymerase Chain Reaction (PCR) technique, on the other hand, allows for the exponential enzymatic amplification of selected HIV-2 DNA sequences present in clinical specimens. The amplified fragments are then detected by hybridization to an HIV-2 specific labeled probe. As a result, the HIV-2 DNA PCR is a highly sensitive and specific method to detect the presence of HIV-2 proviral DNA in clinical specimens.
The diagnosis of infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional clinical tests. A negative PCR result indicates the absence of HIV-2 proviral DNA at detectable levels in the sample tests and does not exclude diagnosis of disease.
The diagnosis of infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional clinical tests. A negative PCR result indicates the absence of HIV-2 proviral DNA at detectable levels in the sample tests and does not exclude diagnosis of disease.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
