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Epstein Barr Virus DNA, Qualitative, Real-Time PCR
Test Code34179
CPT Codes
87798
Preferred Specimen
1 mL whole blood or plasma collected in an EDTA (lavender-top) tube, ACD-B (yellow-top), PPT-potassium EDTA (white-top) tube, or
Serum, or
CSF submitted in a sterile plastic leak-proof container, or
3mm fresh tissue submitted in a sterile plastic leak-proof container
Serum, or
CSF submitted in a sterile plastic leak-proof container, or
3mm fresh tissue submitted in a sterile plastic leak-proof container
Minimum Volume
0.3 mL
Other Acceptable Specimens
Bronchoalveolar lavage or eye fluid collected in a sterile plastic leak-proof container
Instructions
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.
Tissue: Collect in a sterile, plastic container and transport frozen.
Avoid repeated freezing and thawing of specimens. Do not freeze whole blood.
Tissue: Collect in a sterile, plastic container and transport frozen.
Avoid repeated freezing and thawing of specimens. Do not freeze whole blood.
Transport Temperature
Refrigerated (cold packs)
Tissue: Frozen
Tissue: Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood • Heparin whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Report Available
1 to 1 Day(s)
Clinical Significance
Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.
