HLA-B27 Antigen

Test Code

528

CPT Codes
86812<br /> **This test code is for non-New York patient testing. For New York patient testing, use test code 93099.** <br /> Please Note: Per NY requirements, viability studies will be performed for NY patient specimen received more than 48 hours after collection.**

Preferred Specimen

5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume

1 mL

Other Acceptable Specimens

EDTA (lavender-top) tube • ACD-A or ACD-B (yellow-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lithium heparin& (green-top) tube • Clotted • Received frozen

Methodology
Flow Cytometry (FC)

Setup Schedule

Sun, Tue-Sat

Report Available

2 to 4 Day(s)

Clinical Significance

HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.