DIRECT ANTIGLOBULIN TEST W/REFLEX

Test Code

LAB274

Quest Code

36668

CPT Codes
86880

Includes

If Direct Antiglobulin is positive, then Anti C3 and Anti IgG will be performed at an additional charge (CPT code(s): 86880 x2).

Preferred Specimen

5 mL whole blood collected in an EDTA (pink-top) tube

Minimum Volume

5 mL

Other Acceptable Specimens

Whole blood collected in: ACD (yellow-top) tube, EDTA (lavender-top) tube or Cord blood collected in a red top tube (no gel)

Instructions

If submitting with a CBC, HbA1c, or any other test requiring an EDTA (lavender-top) tube, please submit a separate tube for this test

Transport Container

EDTA (pink-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Grossly lipemic • Serum separator tube (SST) • Clotted specimen• Grossly icteric

Methodology
Agglutination

Setup Schedule

a.m. Mon-Fri

Report Available

4 days

Reference Range

Negative

Clinical Significance

The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.

Performing Laboratory

Quest Diagnostics-Lenexa

10101 Renner Blvd

Lenexa , KS 66219-9752

Last Updated: March 23, 2022

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.