STOOL CULTURE W/SHIGA TOXIN

Test Code

LAB223

Quest Code

32114

CPT Codes
87045, 87427, 87449

Includes

Shiga Toxins, EIA with Reflex to E. coli O157, Culture
Salmonella and Shigella, Culture
Campylobacter species Antigen, EIA

Shiga Toxins, EIA with Reflex to E. coli O157, Culture: If Shiga Toxin, EIA is Detected, then Escherichia coli O157, Culture will be performed at an additional charge (CPT code(s): 87046).

Salmonella and Shigella, Culture: If culture is positive, an identification will be performed at an additional charge (CPT code(s): 87077).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87186).

Preferred Specimen

1 gram stool collected in a modified Cary-Blair transport medium

Minimum Volume

1 gram

Instructions

Collect stool in a clean, dry container and then transfer to an enteric transport vial containing modified Cary-Blair transport medium

Transport Container

Cary-Blair stool culture transport medium

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 4 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Dry rectal swab • Raw stool not in transport medium • Specimens in transport medium other than those listed as acceptable • Modified Cary-Blair with a yellow-colored solution, indicating the sample has exceeded the buffering capacity

Methodology
Bacterial Culture • Aerobic Isolation and Identification Procedures • Broth Enhanced Enzyme Immunoassay • Immunoassay (IA)

Reference Range

See Laboratory Report

Clinical Significance

Salmonella, Shigella, and Campylobacter are considered gastro-intestinal pathogens. Identification of these organisms is important for treatment and infection control. The Shiga toxin assay detects toxins produced by enterohemorrhagic E. coli and other enteric organisms which have been isolated from patients who have hemorrhagic colitis with or without hemolytic-uremic syndrome (HUS).

Performing Laboratory

Quest Diagnostics-St.Louis

11636 Administration Drive

Maryland Heights, MO 63146-3534

Last Updated: September 2, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.