HSV BY PCR, CSF

Test Code
LAB3477


Quest Code
19502


CPT Codes
87530 (x2)

Preferred Specimen

1 mL CSF, vitreous, pericardial, pleural, or amniotic fluid collected in a sterile, leakproof container OR
1 mL serum OR
1 mL plasma collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
1 mL whole blood collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube

Note: Do not freeze whole blood.



Minimum Volume
0.3 mL


Other Acceptable Specimens
1 mL bronchoalveolar lavage or bronchial wash


Instructions
Note: Do not freeze whole blood


Transport Container
Sterile, plastic, leak-proof container


Transport Temperature
Whole blood: Refrigerated (cold packs)
All other specimen types: Frozen


Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days


Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Daily


Reference Range
HSV 1, DNA, QN, PCRNot Detected
HSV 2, DNA, QN, PCRNot Detected


Clinical Significance
The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042



Last Updated: August 13, 2021


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.