NRAS Mutation Analysis: 1003867

Message
Pathology report must be provided for tissue sample.

Test Code
NRASPY or 1003867

Alias/See Also
N-RAS

CPT Codes
81311

Instructions
Preferred specimen type: Formalin fixed paraffin embedded tissue.
 
Alternate specimen: Lavender EDTA or Sodium heparin whole blood or bone marrow.

Transport Container
Parrafin Block: sample source and block ID are required.  A pathology report must be submitted.
 
Alternate:  Cut one 3-5 um tissue section for H&E stain (or provide stained H&E slide). Cut 8 (Minimum 4) slides 10 um sections on charged (+) slides. The slide should not be baked or melted. Provide pathology report.
 
Alternate:  5 mL  whole blood (Min: 3 mL) or 3 mL (Min: 1 mL) bone marrow aspirate in an EDTA (lavender-top) or Sodium heparin ( green-top) tube.

Transport Temperature
Tissue: Room temperature.
Whole blood or bone marrow: Refrigerated.

Specimen Stability
Formalin-fixed paraffin embedded tissue:
Room temperature: 5 years; Refrigerated: 5 years; Frozen: Unacceptable

Whole blood and bone marrow:
Room temperature: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Methodology
Polymerase Chain Reaction, Sequencing

Setup Schedule
Sunday - Saturday

Report Available
5 days  (From receipt at performing laboratory)

Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Not detected.

Clinical Significance
NRAS mutations have been described in a variety of cancers including 3-5% of colorectal carcinomas, 10-20% of melanomas and 10% of acute myeloid leukemias. Mutations leading to an amino acid substitution at positions 12, 13, 61 and 146 of NRAS are the most common in naturally occurring neoplasms.

Performing Laboratory
Quest Diagnostics



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.