FLT3  ITD MRD Testing by NGS, Ultra-sensitive : 1016792

Message
Indications for testing:
Identify tumor-specific markers for post-treatment monitoring
Monitor and evaluate disease recurrence

Test Code
FLTMRD or 1016792

Alias/See Also
FLT3, MRD, Minimal residual disease (MRD) detection

CPT Codes
0046U

Instructions
Whole blood or bone marrow in EDTA lavender top tube or ACD  yellow top tube.   EDTA is preferred for MRD testing.
Alternative specimen: 700-2000 ng previously isolated DNA.

Transport Container
Transport 5 mL whole blood (Min: 4 mL) or 3 mL bone marrow  (Min: 1 mL) or alternative specimen type.

Transport Temperature
Refrigerated.

Specimen Stability
Room temperature:  72 hours; Refrigerated:  7 days; Frozen:  Unacceptable
Isolated DNA:  Ambient: 72 hours; Refrigerated: Indefinitely; Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted or hemolyzed specimens.

Methodology
Next Generation Sequencing

Setup Schedule
Monday - Saturday

Report Available
10 days  (From receipt at performing laboratory)

Limitations
FLT3 Mutation Testing is performed pursuant to patents licensed from Takara Bio Inc. of Kusatsu (Shiga prefecture), Japan.

Reference Range
By report.

Clinical Significance
Used to track and identify previously detected FLT3 ITD mutations in post treatment follow up samples, a multiplex master mix targeting the juxtamembrane domain of the FLT3 gene is used to amplify DNA extracted from a patient sample.

Performing Laboratory
LabPMM



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.