Vitamin D, 25(OH) by LC/MS/MS : 1000988

Test Code
VITD or 1000988

Alias/See Also
25-Hydroxy D2; 25-Hydroxy D3; 25-Hydroxy Vitamin D; 25-Hydroxycholecalciferol; 25-Hydroxyergocalciferol; 25-OH Vitamin D; Calcidiol; D2; D3; total 25-Hydroxy Vitamin D; Vitamin D Assay; 25-hyrdroxy vitamin D2; 25-hyrdroxy vitamin D3; Vitamin D, 25-Hydrox

CPT Codes

Panel includes: D2, D3, and Total.

Serum separator tube or a plain red top tube.

Transport Container
Centrifuge the serum separator tube and remove serum from gel within 48 hours; if a plain red top tube, centrifuge after clotting and aliquot serum (Min. 1 mL) into a standard transport tube.

Transport Temperature

Specimen Stability
After separation from cells: Ambient: 21 days; Refrigerated: 21 days; Frozen: 21 days

LC/MS/MS - Multiplex

Setup Schedule
Monday - Saturday

Report Available
1-2 days

This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test
is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.

Reference Range
Effective 3/6/2019
Vitamin D25-hydroxy : 30-100 ng/mL
Vitamin D2: by report
Vitamin D3: by report

Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. Deficiency of 25-OH vitamin D can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia.

This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the vitamin and 25OH D2 - the analog form used to treat 25OH Vitamin D3 deficiency.

Performing Laboratory
med fusion

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.