Varicella-Zoster Virus (VZV), Qualitative by PCR, Non-Blood : 1004209

Test Code
VZVNBL or 1004209

Alias/See Also
Chickenpox by PCR; Herpes Zoster by PCR; Varicella-zoster Virus Detection by Real-Time PCR; VZV Dermal by PCR; VZV Detection by Real-Time PCR

CPT Codes

The following specimen types are acceptable:

CSF (Cerebrospinal Fluid); Body Fluids (Amniotic Fluid, Ocular); Dermal sore/lesion; Bone Marrow; Upper respiratory specimens (nasal/throat swabs); Lower respiratory specimens (BAL, bronchial washings, tracheal secretions); and Tissue.

Transport Container
Submit 1 mL CSF or Body Fluid (Min. 0.5 mL) in a sterile plastic screw capped container or tube.

Submit 2 mL Bone Marrow (Min. 0.5 mL) in a lavender top EDTA tube.

Submit Upper Respiratory specimens collected using polyester-tipped, Dacron or rayon-tipped collection swabs with plastic shafts in M4 or M5 Viral Transport Media.

Submit Lower Respiratory specimens in M4 or M5 Viral Transport Media.

Submit dry swab or swab in M4 or M5 Viral Transport Media.for dermal sore/lesion.

Submit Fresh and Frozen tissue in sterile container. Fresh tissue must be put in RPMI media. Frozen tissue must be in OCT compound. Submit Paraffin embedded tissue in sterile biohazard plastic bag. Submit slides in slide holder.

Note: Do not centrifuge any specimen type.

Transport Temperature
Bone Marrow, CSF, Body Fluid, Respiratory, Dry swab or Fresh Tissue: Refrigerated

Frozen Tissue: Frozen

Paraffin Embedded Tissue: Ambient or on ice pack in summer

Slides: Ambient

Specimen Stability
CSF, Body Fluids: Ambient: 8 hours; Refrigerated: 5 days; Frozen: 1 month.

Bone Marrow: Ambient: 8 hours; Refrigerated: 5 days; Frozen: Unacceptable

Paraffin Embedded Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Dry swab or Fresh Tissue: Refrigerated: 3 days; Frozen: 3 months

Qualitative Real-Time Polymerase Chain Reaction (PCR)

Setup Schedule
Monday & Wednesday & Friday

Report Available
1-4 days

Reference Range
Target Not Detected (TND)

Clinical Significance
Varicella-Zoster Virus (VZV) is a member of the Herpesviridae family that causes varicella (chickenpox) as primary infection and herpes zoster (shingles) upon reactivation. Chickenpox is a childhood infection presented as generalized vesicular rash on the dermis in normal children, usually before 10 years of age. After the primary infection, the virus persists in latent form in the dorsal ganglion and may retrograde in elderly or immunocompromised host to cause shingles that presents as a unilateral vesicular eruption, generally in a dermatomal distribution. VZV DNA in skin lesions, cerebrospinal fluid (CSF) and specimens from respiratory tract specimens permits rapid and sensitive patient testing and usually indicates active, not latent, infection. A negative result does not exclude the possibility of VZV infection. This assay is only to be used for patients with a clinical history and symptoms consistent with VZV infection, and must be interpreted in the context of the clinical picture. This test is not used to screen asymptomatic patients.

Performing Laboratory
med fusion

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.