Neisseria gonorrhoeae RNA by TMA, Anal-Rectal : 1004859

Test Code
GCREC or 1004859

Alias/See Also
Neisseria gonorrhoeae by Nucleic Acid Amplification Test (NAAT); Anal-Rectal gonococcal infections

CPT Codes
87591

Instructions
Anal-Rectal cytology sample can be collected using either PreservCyt® (ThinPrep, Client Supply # P70098) or SurePath™ (Client Supply # P490527) fixative. Rectal specimens can also be collected with Aptima Unisex (Client Supply # 11071) or Aptima Vaginal (Client Supply # B30079) Swab Collection Kits, and submitted in the APTIMA® swab specimen transport tube.

Instructions for Anal-rectal cytology specimen collection: Collect anal-rectal cytology specimen using a Dacron swab. It is important to use Dacron® and not a cotton swab, as cells tend to cling to cotton and do not release easily into cytology collection fluids. Moisten the swab in tap water and insert as 2-4 cms into the anal canal. Slowly rotate the swab in one direction with gentle pressure on the walls as the swab is slowly being withdrawn. Care should be taken to ensure the transition zone is sampled. Vigorously rotate the swab in the LCM solution while pushing against the wall of the vial. Swirl the swab vigorously to release additional material. Discard the swab. Tighten the cap on the container so that the torque line on the cap passes the torque line on the vial.

Anal-rectal cytology specimen collected using Brush/Spatula or Broom-like collection devices is also acceptable. Insert the collection device 2-3 cms into the anal canal. Slowly retract the device with a twisting motion. Break off the tip and drop in vial. Use the spatula to perform a sweep of the anus. Break off the spatula tip and drop in vial.

Instructions for Anal-rectal swab specimen collection:Insert the vaginal or blue-shafted unisex swab 2-4 cms into the rectum and gently rotate for 10 to 30 seconds to sample the mucosal surfaces. Withdraw the swab carefully. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Carefully break the swab shaft at the scoreline; use care to avoid splashing of the contents. Recap the swab specimen transport tube tightly.

Note: Do not use the white-shafted swab provided in the vaginal swab collection kit.

Transport Container
Submit Anal-Rectal cytology sample in PreservCyt® vial or SurePath™ vial or 2 mL (Min. 1 mL) aliquot of vial (Client Supply # B11700).

Submit Aptima Unisex (Client Supply # 11071) or Aptima Vaginal Swab Client Supply # B30079) in the APTIMA® swab specimen transport tube.

Transport Temperature
Ambient.

Specimen Stability
Swab: Ambient: 60 days; Refrigerated: 60 days; Frozen: 12 months

PreservCyt®: Ambient: 6 months; Refrigerated: 6 months; Frozen: Unacceptable

SurePath™: Ambient: 24 hours; Refrigerated: 4 weeks; Frozen: Unacceptable

Methodology
Nucleic Acid Amplification Test (NAAT) based on Transcription Mediated Amplification (TMA) of RNA; Gen Probe APTIMA Combo 2

Setup Schedule
Monday - Friday

Report Available
1-4 days

Reference Range
Target Not Detected

Clinical Significance
Chlamydia trachomatis and Neisseria gonorrhoeae may infect the anal-rectal canal of sexually active individuals. Changing patterns of human sexual activity make extragenital C. trachomatis and N. gonorrhoeae infections more common in men. Because extragenital infections are asymptomatic, routine annual screening of extragenital sites in MSM is recommended. Detection of these organism may be important for determining the risk for disease progression in infected individuals or transmission amongst partners.

NAATs in general have better overall sensitivity, specificity, and specimen stability in transport than any of the non-molecular tests.

Performing Laboratory
med fusion



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.