Adenovirus, Quantitative by PCR : 1005000

Message
For tissue specimens, please provide most recent pathology report.

Test Code
ADVQT or 1005000

CPT Codes
87799

Instructions
The following specimen types are acceptable:

Peripheral Blood; CSF (Cerebrospinal Fluid); Body Fluid; Urine; Upper respiratory specimens (nasal, nasopharyngeal and throat swabs; Lower respiratory specimens (BAL, bronchial washings, tracheal secretions); and Tissue.

Transport Container
Submit 5 mL Blood (Min. 1 mL) in a lavender top EDTA tube.

Submit 1 mL CSF (Min. 0.5 mL) or Body Fluid (Min. 2 mL) in a sterile plastic screw capped container or tube.

Submit Upper Respiratory specimens collected using polyester-tipped, Dacron or rayon-tipped collection swabs with plastic shafts in M4 or M5 Viral Transport Media.

Submit Lower Respiratory specimens in M4 or M5 Viral Transport Media.

Submit Fresh and Frozen tissue in sterile container. Fresh tissue must be put in RPMI media. Frozen tissue must be in OCT compound. Submit Paraffin embedded tissue in sterile biohazard plastic bag. Submit slides in slide holder.

Note: Do not centrifuge any specimen type.

Transport Temperature
Blood, CSF, Body Fluid, Respiratory or Fresh Tissue: Refrigerated

Frozen Tissue: Frozen

Paraffin Embedded Tissue: Ambient or on ice pack in summer

Slides: Ambient

Specimen Stability
Blood: Ambient: 8 hours; Refrigerated: 5 days; Frozen: Unacceptable

CSF, Body Fluid, Urine and Respiratory specimens: Ambient: 8 hours; Refrigerated: 5 days; Frozen: 1 month

Paraffin embedded tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Fresh tissue: Refrigerated: 24 hours; Frozen: 3 months

Methodology
Argene Quantitative Real-Time Polymerase Chain Reaction (PCR)

Setup Schedule
Monday - Saturday

Report Available
1-3 days

Limitations
Laboratory testing should be interpreted in the context of additional clinical findings. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration
(FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments
of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.

Reference Range
Target Not Detected (TND)

250 to 10,000,000,000 copies/mL (2.40 to 10.00 log copies/mL)

Respiratory and Tissue specimens will be reported qualitative ONLY.


Clinical Significance
Adenoviruses (AdV) are medium-sized, non-enveloped DNA viruses. At least seven human AdV species (A-G) have been described, comprising at least 60 types/serotypes. AdV typically infect children and military recruits either during a local outbreak or as endemic infections and can cause respiratory, ocular or gastrointestinal disease. AdV infections are common, have a worldwide distribution and can occur throughout the year. AdV have been increasingly recognized as significant viral pathogens and can result in high morbidity and mortality among immune-compromised patients. Clinical manifestations in immunocompromised patients include pneumonia, hepatitis, hemorrhagic cystitis, colitis, pancreatitis, meningoencephalitis and disseminated disease, depending on the underlying disease, affected organ system, patient age, and virus serotype. Amplification and detection of the viral genome by Real Time PCR is highly-sensitive and rapid assay. The ADVQT assay enables the detection (NOT the differentiation) of all the AdV serotypes listed below:
• AdV A: 12, 18, 31
• AdV B: 3, 7, 11, 14, 16, 21, 34, 35, 50
• AdV C: 1, 2, 5, 6
• AdV D: 8 to 10, 13, 15, 17, 19, 20, 22 to 30, 32, 33, 36 to 39, 42 to 49, 51
• AdV E: 4
• AdV F: 40, 41
• AdV G: 52
• Unclassified AdV: 53 to 60

Performing Laboratory
med fusion



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.