MYD88, Mutation Analysis : 91771

Test Code
MYD88Q or 91771

Alias/See Also
MYD88 L265P

CPT Codes
81305

Instructions
Whole blood: Lavender (EDTA), Sodium heparin (green-top) tube, or ACD-B (yellow-top) tube or Bone marrow: Lavender (EDTA) or Formalin-fixed, paraffin-embedded tissue or slides.

Transport Container
Submit: 5 mL (Min. 2 mL) whole blood, 2 mL (Min. 0.5 mL) bone marrow, tissue block, or 10 (Min. 6 ) tissue slides.

Transport Temperature
Refrigerated.

Specimen Stability
Whole blood/Bone marrow:
Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Formalin fixed paraffin embedded tissue/Slides
Ambient: Indefinite; Refrigerated: Indefinite; Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen.

Methodology
DNA Sequencing

Setup Schedule
Sunday - Saturday

Report Available
5 days (From receipt at performing laboratory)

Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Not detected.

Clinical Significance
L265P mutation in the MYD88 gene is found in approximately 90% of WaldenstrÖm macroglobulinemia and IgM-expressing lymphoplasmacytic lymphoma (LPL). There is a low incidence of L265P MYD88 mutation in other systemic CD5-negative B-cell lymphoproliferative disorders including atypical chronic lymphocytic leukemia, nodal marginal zone lymphoma (MZL), splenic MZL and mucosa-associated lymphoid tissue (MALT)-type MZL.
This PCR-based DNA pyrosequencing assay sensitively detects the L265P MYD88 mutation and can be used to help diagnose WaldenstrÖm macroglobulinemia or IgM-expressing lymphoplasmacytic lymphoma and to help in stratifying or subclassifying patients with IgM monoclonal gammopathy.

Performing Laboratory
Quest Diagnostics



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.