Vitamin D, 1,25-Dihydroxy : 16558

If sample is submitted with less than 0.5 mL and needs to be repeated the sample will be cancelled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".

Test Code
VTD125 or 16558

CPT Codes

Includes Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2.

Serum separator tube.

Transport Container
Allow blood to clot at room temperature. Centrifuge the serum separator tube and transport 1 mL serum. (Min: 0.5)

Transport Temperature

Specimen Stability
Ambient: 28 days; Refrigerated: 28 days; Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, Lipemia, Icteric specimens, Plasma.

Liquid Chromatography/Tandem Mass Spectrometry

Setup Schedule
Sunday - Saturday

Report Available
3 days (From receipt at performing laboratory)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Vitamin D, 1,25 (OH)2, Total
1-9 Years 31-87 pg/mL
10-13 Years 30-83 pg/mL
14-17 Years 19-83 pg/mL
Adult 18-72 pg/mL
Vitamin D3, 1,25 (OH)2 Not available
Vitamin D2, 1,25 (OH)2 Not available

Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for informational purposes only.

Clinical Significance
This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypercalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes.

Performing Laboratory
Quest Diagnostics

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.