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QuantiFERON®-TB Gold
Test CodeLAB5070 1230005070
Alias/See Also
QFT
TB
QUANTIFERON
TB
QUANTIFERON
CPT Codes
86480
Includes
QFT RESULT
QFT NIL
QFT TB1
QFT TB2
QFT MITOGEN
QFT NIL
QFT TB1
QFT TB2
QFT MITOGEN
Minimum Volume
4
Instructions
Collect minimum 4 mL in Green LiHep tube (no gel)
OR
1 mL whole blood collected in each of the following tubes:
QFT-Nil control (gray cap, white or gold ring) tube
QFT-TB1 antigen (green cap, white or gold ring) tube
QFT-TB2 antigen (yellow cap, white or gold ring) tube
QFT-Mitogen control (purple cap, white or gold ring) tube
For each patient, collect 1 mL of blood by venipuncture directly into each of the 4 QFT-PLUS blood collection tubes.
If a "butterfly needle" is being used, a "purge" tube should be used to ensure that the tubing is filled with blood prior to the QFT-PLUS tubes being used.
Immediately after filling the tubes, shake them 10 times just firmly enough to ensure the entire surface of the tube is coated with blood, to solubilize antigens on tube walls.
OR
1 mL whole blood collected in each of the following tubes:
QFT-Nil control (gray cap, white or gold ring) tube
QFT-TB1 antigen (green cap, white or gold ring) tube
QFT-TB2 antigen (yellow cap, white or gold ring) tube
QFT-Mitogen control (purple cap, white or gold ring) tube
For each patient, collect 1 mL of blood by venipuncture directly into each of the 4 QFT-PLUS blood collection tubes.
If a "butterfly needle" is being used, a "purge" tube should be used to ensure that the tubing is filled with blood prior to the QFT-PLUS tubes being used.
Immediately after filling the tubes, shake them 10 times just firmly enough to ensure the entire surface of the tube is coated with blood, to solubilize antigens on tube walls.
Transport Container
QFT Collection
Transport Temperature
Room Temperature
Specimen Stability
Lab processing:
Collect 1 mL of blood by venipuncture directly into each of the 4 QFT-PLUS blood collection tubes. These tubes should be at 17-25° C at the time of draw.
Immediately after filling the tubes, shake them 10 times just firmly enough to ensure the entire surface of the tube is coated with blood, to solubilize antigens on tube walls.
Each of the four tubes must be transferred to a 37° C +/- 1° C incubator as soon as possible and within 16 hours of collection for 16-24 hours. If the blood is not incubated immediately after collection, re-mixing of the tubes by inverting 10 times must be performed immediately prior to incubation. The specimen rack should sit off the floor of the incubator and the tubes should be upright.
Centrifuge for 15 min at 2000-3000 RCF(G).
Collect 1 mL of blood by venipuncture directly into each of the 4 QFT-PLUS blood collection tubes. These tubes should be at 17-25° C at the time of draw.
Immediately after filling the tubes, shake them 10 times just firmly enough to ensure the entire surface of the tube is coated with blood, to solubilize antigens on tube walls.
Each of the four tubes must be transferred to a 37° C +/- 1° C incubator as soon as possible and within 16 hours of collection for 16-24 hours. If the blood is not incubated immediately after collection, re-mixing of the tubes by inverting 10 times must be performed immediately prior to incubation. The specimen rack should sit off the floor of the incubator and the tubes should be upright.
Centrifuge for 15 min at 2000-3000 RCF(G).
Performing Laboratory
MWH LAB
