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VRE SURVEILLANCE BY PCR
Test CodeLAB5062 1230102124
Alias/See Also
VRECP
VRE SCREEN
SURVEILLANCE
VANC SCREEN
VRE SCREEN
SURVEILLANCE
VANC SCREEN
CPT Codes
87500
Includes
VANCOMYCIN RESISTANT ENTEROCOCCUS PRESENCE IN UNSPEC SP
Preferred Specimen
Perirectal swab
Instructions
Specimen source is required on request form for processing.
Specimen Requirements:
Preferred Source: Perirectal
Preferred Collection/Container: Red top double swab culturette
Alternative Acceptable Source: Rectal
Collection Process
Perirectal Specimens:
1. A double tip swab (red top) is required for collection.
2. Press buttocks apart to expose perianal region. Using both swabs, fully swab around the perianal surface.
3. Return swab to tube to ensure specimen preservation.
4. Label swab with patient’s name (first and last), Social Security number or date of birth (inpatients: medical record number), date and time of collection, collector’s initials, and type of specimen.
5. Maintain sterility and forward promptly at ambient temperature only. Do not refrigerate.
Rectal Specimens:
1. A double tip swab (red top) is required for collection.
2. Carefully insert the swab 2.5 cm beyond the anal sphincter (so that the swab tip is no longer visible) and gently rotate 3 times to endure uniform sample on both swabs.
3. Return swab to tube to ensure specimen preservation.
4. Label swab with patient’s name (first and last), Social Security number or date of birth (inpatients: medical record number), date and time of collection, collector’s initials, and type of specimen.
5. Maintain sterility and forward promptly at ambient temperature only. Do not refrigerate.
Rejection Criteria
Unacceptable Source: Source other than listed above.
Unacceptable collection: Swab grossly contaminated with fecal material (see Swab Examples image below). Incorrect swab collection device, improperly labeled, unlabeled
Transport Container
STERILE SWAB
Transport Temperature
Room Temperature
Specimen Stability
Storage: Ambient <24 hours. Refrigerated specimens at 2° to 8° C are stable for 24 hours up to 5 days.
Transport: Ambient or 2° to 8° C
Transport: Ambient or 2° to 8° C
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejection Criteria
Unacceptable Source: Source other than listed above.
Unacceptable collection: Swab grossly contaminated with fecal material (see Swab Examples image below). Incorrect swab collection device, improperly labeled, unlabeled
Reference Range
Not Detected
Clinical Significance
The Cepheid Xpert® vanA Assay performed in the GeneXpert® Dx System is qualitative in vitro diagnostic test designed for rapid detection of the vanA gene
sequence associated with vancomycin resistance in bacteria obtained from rectal swab specimens from patients at risk for intestinal colonization with vancomycin resistant bacteria.
The test utilizes automated real-time polymerase chain reaction (PCR) to detect the vanA gene that is frequently associated with vancomycin resistant enterococci (VRE). The Xpert vanA Assay is intended to aid in the recognition, prevention, and control of vancomycin-resistant organisms that colonize patients in healthcare settings
sequence associated with vancomycin resistance in bacteria obtained from rectal swab specimens from patients at risk for intestinal colonization with vancomycin resistant bacteria.
The test utilizes automated real-time polymerase chain reaction (PCR) to detect the vanA gene that is frequently associated with vancomycin resistant enterococci (VRE). The Xpert vanA Assay is intended to aid in the recognition, prevention, and control of vancomycin-resistant organisms that colonize patients in healthcare settings
Performing Laboratory
MWH LAB
