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MRSA Surveillance by PCR
Test CodeLAB1368 1230101558
Alias/See Also
METHICILLIN RESISTANT STAPH AUREUS
MRSPCR
SURVEILLANCE BY PCR
MRSAPCR
MRSA
MRSA SCREEN
MRSA PCR
SURVEILLANCE
METHICILLIN
RESISITANT
MRSPCR
SURVEILLANCE BY PCR
MRSAPCR
MRSA
MRSA SCREEN
MRSA PCR
SURVEILLANCE
METHICILLIN
RESISITANT
CPT Codes
87641
Includes
MRSA DNA
Preferred Specimen
Nasal swab
Other Acceptable Specimens
Nares
Instructions
Specimen source is required on request form for processing:
Specimen Requirements:
Preferred Source Nasal
Preferred Collection/Container Red top double swab culturette
Alternative Acceptable Source Nares
Acceptable Collection:
BD Liquid Amies Elution Swab (E-Swab); Copan Liquid Amies Elution Swab (ESwab); Cepheid nasal sample collection device (dual swab in liquid Stuart media); or Copan swab and transport systems (LQ Stuart or LQ Amies) (scored swabs only)
Collection Instructions:
1. Ask the patient to blow their nose prior to collection.
2. Use both swabs for both right and left nares.
Note: to avoid specimen contamination, do not touch swab tips to anything other than the inside of the nostril.
3. Insert the dry swabs 1-1.5 cm into the nostril. Swabs MUST stay attached to red cap. Do NOT insert swabs more than 1-1.5 cm.
4. Rotate the swab for 3 seconds against the nasal mucosa. Apply slight pressure on the outside of the nose with your finger to ensure good contact.
5. Repeat the process on the other nostril with the same swabs.
6. Remove and discard the cap on the transport tube then Insert the swabs back into the transport tube. The swabs should go all the way into the transport container. Tightly cap the container.
7. Label the transport container with patient label or label swab with patient’s name (first and last), Social Security number or date of birth (inpatients: medical record number), date and time of collection, collector’s initials, and type of specimen.
8. Maintain sterility and forward promptly at ambient temperature only. Do not refrigerate.
Specimen Rejection:
Unacceptable Source: Source other than Nares, Nasal
Unacceptable collection: Incorrect swab collection device, improperly labeled, unlabeled
Transport Container
STERILE SWAB
Transport Temperature
Room Temperature
Specimen Stability
Storage:
Ambient <24 hours. Refrigerated specimens at 2° to 8° C are stable for 24 hours up to 7 days.
Transport:
Ambient or 2° to 8° C
Ambient <24 hours. Refrigerated specimens at 2° to 8° C are stable for 24 hours up to 7 days.
Transport:
Ambient or 2° to 8° C
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen Rejection:
Unacceptable Source: Source other than Nares, Nasal
Unacceptable collection: Incorrect swab collection device, improperly labeled, unlabeled
Reference Range
MRSA DNA- Not Detected
Clinical Significance
Useful for the direct detection of colonization of nares by MRSA and is intended to aid in the prevention and control of MRSA infections in healthcare settings.
The Xpert MRSA NxG test is a qualitative in vitro diagnostic test intended for the detection of methicillin-resistant Staphylococcus aureus (MRSA) DNA directly from nasal swabs in patients at risk for nasal colonization. The test utilizes automated real-time polymerase chain reaction (PCR) for the amplification of MRSA-specific DNA Targets and fluorogenic target-specific hybridization probes for the real-time detection of the amplified DNA.
The Xpert MRSA NxG test is a qualitative in vitro diagnostic test intended for the detection of methicillin-resistant Staphylococcus aureus (MRSA) DNA directly from nasal swabs in patients at risk for nasal colonization. The test utilizes automated real-time polymerase chain reaction (PCR) for the amplification of MRSA-specific DNA Targets and fluorogenic target-specific hybridization probes for the real-time detection of the amplified DNA.
Performing Laboratory
MWH LAB
