Cytomegalovirus, Conventional and Rapid, Culture

Test Code
CMV-CUL(2627)

Quest Code
2627

CPT Codes
87252, 87254

Preferred Specimen
3 mL bronchial lavage/wash, or tracheal aspirates, or nasopharyngeal swabs/aspirate/wash, or throat swab, or urine, or tissue/biopsy, or saliva swab, or urogenital swab, or amniotic fluid collected in VCM or equivalent or sterile screw-cap container

Minimum Volume
0.5 mL

Other Acceptable Specimens
Bone marrow collected in heparin (green-top) tube

Instructions
To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8°C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in VCM or equivalent should be frozen at -70°C or colder and transported on dry ice. Storage or transport at -20°C is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.

Tissue must be kept moist.

Note: PCR is preferred test for CSF and whole blood (preferred specimen: 1 mL CSF submitted in a sterile, leak-proof container without transport media, 1 mL whole blood in EDTA)

Transport Temperature
VCM specimens: Frozen -70°C
Other specimens: Refrigerated (cold packs)

Specimen Stability
VCM or equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days

Raw (unpreserved) specimens
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable

Bone marrow heparin
Refrigerated: 72 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Wooden-shaft • Alginate swabs • CSF • eSwab • Bacterial transport media • Stool specimens • Blood

Methodology
Spin Amplification with Monoclonal Antibody Detection • Tissue Culture with Monoclonal Antibody Conventional Identification

Setup Schedule
Set up: Daily; Report available: 4-23 days

Reference Range
Rapid Culture Result Not Isolated
Tube Culture Result Not Isolated


Clinical Significance
CMV infections are common and usually asymptomatic. In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV may cause birth defects in a minority of infected newborns. Rapid culture provides results quickly in order to initiate therapy but may be negative in certain circumstances. Conventional culture may take more than 3 weeks.

Performing Laboratory
Quest Diagnostics Infectious Disease, Inc.
33608 Ortega Highway, Bldg B-West Wing
San Juan Capistrano, 92675-2042



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.