OSMOTIC FRAGILITY
 

Message
MAYO TEST CODE:FRAG

Only draw this test Monday-Wednesday

Follow this link to print out form that is to be sent with the specimen.
https://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf

Test Code
OSMOTIC

Alias/See Also
FRAGILITY, OSMOTIC (RBC)
RBC Fragility, Erythrocytes
Red Cell Fragility

CPT Codes
85557

Preferred Specimen

Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
 

Minimum Volume
2 mL lavender top EDTA whole blood

Instructions
Patient:
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
2. Send specimen in original tube. Do not aliquot.
3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
4. Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.
Additional Information: Patient's age and sex are required.
 
Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
2. Label clearly on outermost label normal control.
3. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
4. Send specimen in original tube. Do not aliquot.
5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

Transport Temperature
Refrigerated

Specimen Stability
72 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, clotted or frozen specimens

Methodology
Osmotic Lysis

Report Available
5 days

Clinical Significance
Used for the evaluation of suspected hereditary speherocytosis and associated hemolytic anemia

Performing Laboratory
MAYO



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.