Test Code
CORT-SAL (19897)

Quest Code

CPT Codes

Preferred Specimen
0.5 mL saliva collected in a Super SAL™ Universal Saliva Collection Kit

Minimum Volume
0.2 mL

1. Do not eat, drink, smoke, or use oral hygiene products for at least 30 minutes, then rinse the mouth with water and discard. Please wait at least 5 minutes after this rinse to start the collection procedure.
2. During this time review the detailed instructions on the SUPER SAL™ Saliva collection Kit insert, particularly the illustrations showing the appearance of the Sample Volume Adequacy Indicator (SVAI) before and after sample collection (Figure 3).
3. Place contents on a clean and dry surface.
4. Firmly attach the Collection Tube (E) provided to the base (D) of the Compression tube (C) (Figure 1). In preparation for saliva collection, pool saliva in the mouth. Sample collection may now begin.
5. Place the tip of the pad of the Collector (A) where saliva has pooled (Figure 2).
6. The Collector (A) may be removed from the mouth periodically to read the SVAI, but resume collection immediately afterward. Collect until the appearance of the SVAI (B) changes from white to fully red (Figure 3). The collection procedure should last approximately 1-5 minutes.
7. Place the white absorbent pad end into the Compression Tube (C) holding the Collector (A) in an upright and vertical position and firmly push the plunger downwards to transfer saliva from the absorbent pad into the Collection Tube (E). Push and hold for 30 seconds (Figure 4).
8. Gently remove the Collection Tube (E) from the end of the Compression Tube (C). It is appropriate to remove the Collection Tube (E) during expression if necessary due to foaming. Close the lid on the tube tightly.
9. Label the collected sample immediately, include date and time of collection and store at refrigerated temperatures prior to shipment. For Technical Support, call 1-800-642-4657 ext. 2904.


1. Saliva should be collected at the time(s) prescribed by your doctor.
2. No food or fluids for 30 minutes prior to collection.
3. Do not use any creams, lotions, or steroid inhalers immediately prior to collection.
4. Avoid any activity that can cause your gums to bleed, including brushing and flossing your teeth. Consult with your doctor if this is a chronic problem.

Transport Container
Super SAL™ Universal Saliva Collection Kit

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 21 days
Frozen: 3 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Any tube other than Super SAL™ Universal Saliva Collection Kit

Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Report Available
2 to 6 Day(s)

Reference Range
8-10 AM 0.04-0.56 mcg/dL
Noon-2 PM ≤0.21 mcg/dL
4-6 PM ≤0.15 mcg/dL
10 PM-1 AM ≤0.09 mcg/dL

Performing Laboratory
Quest Diagnostics Nichols Institute San Juan Capistrano
33608 Ortega Hwy
San Juan Capistrano, CA 92675-2042

Additional Information
Collection Instruction Video

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.