Vitamin B6, Plasma

Test Code
B6-VIT (926)

Quest Code
926

Alias/See Also
PYRIDOXINE

CPT Codes
84207

Preferred Specimen
1 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light

Patient Preparation
Overnight fasting is required

Minimum Volume
0.5 mL

Instructions
Separate cells within 6 hours by centrifugation at 2-8° C (2200-2500 rpm, 800-1000g) for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen.

Transport Temperature
Frozen

Specimen Stability

Room temperature: 6 hours
Refrigerated: 12 hours
Frozen -20°: 6 days
Frozen -70°: 42 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Received room temperature • Received refrigerated

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Sun-Fri; Report available: 1-4 days

Reference Range
0-1 year Not established
2-17 years 3.0-35.0 ng/mL
≥18 years 2.1-21.7 ng/mL


Clinical Significance
Test is used to determine vitamin B6 deficiency or overdosage, for monitoring treatment, and to evaluate wellness and health.

Performing Laboratory
Quest Diagnostics Nichols Institute
27027 Tourney Road
Valencia, CA 91355-5386



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.