Vitamin C

Test Code
VITC (929)

Quest Code

CPT Codes

Preferred Specimen
1 mL frozen serum - protected from light

Patient Preparation
Overnight fasting is preferred.
Refrain from eating fruits and from taking vitamin C supplements for 24 hours prior to collection.

Minimum Volume
0.5 mL

Other Acceptable Specimens
Plasma collected in: Sodium heparin (green-top) tube protected from light

Centrifuge within 1 hour of collection. Immediately separate serum from the cells into a clean, plastic transport tube. Freeze. Protect from light.
If unable to centrifuge immediately, sample should be covered with aluminum foil immediately and placed on ice until serum can be separated from cells. Transport to the laboratory frozen.

Transport Temperature

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen -20° C: Unacceptable
Frozen -70° C: 35 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Received room temperature • Received refrigerated • Not protected from light • EDTA plasma • Lithium heparin plasma • Thawed samples

Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Reference Range
Female 0.3-2.7 mg/dL
Male 0.2-2.1 mg/dL

Clinical Significance
Vitamin C is an antioxidant involved in connective tissue metabolism, drug-metabolizing systems, and mixed-function oxidase systems to list a few. Vitamin C deficiency causes scurvy; manifestations include impaired formation of mature connective tissue, bleeding into the skin, weakness, fatigue, and depression.

Performing Laboratory
Quest Diagnostics Nichols Institute
27027 Tourney Road
Valencia, CA 91355-5386

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.