Cortisol, Free, LC/MS/MS, 24-Hour Urine [11280X]

Test Code

Quest Code

CPT Codes

CPT code 81050 may be added at an additional charge for volume measurement

Preferred Specimen
2 mL unpreserved 24-hour urine

Minimum Volume
0.5 mL

Other Acceptable Specimens
Preserved 24-hour urine in 24 mL 6N HCl, or 10 mL conc. glacial acetic acid, or 10 grams boric acid

Record 24-hour urine volume on test requisition and urine vial. Reference ranges do not apply to random urine samples.

Transport Container
Sterile, leak-proof container

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 14 days
Refrigerated: 21 days
Frozen: 3 months

Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Setup Schedule
Set up: Mon-Fri; Report available: 2-6 days

Reference Range
Cortisol, Free, 24-Hr Urine
 Adult 4.0-50.0
   1-4 Years 0.9-8.2 mcg/24 hrs
   5-9 Years 1.0-30.0 mcg/24 hrs
   10-13 Years 1.0-45.0 mcg/24 hrs
   14-17 Years 3.0-55.0 mcg/24 hrs

Creatinine, 24-Hour Urine
<3 Years Not established
3-8 Years 0.10-0.80 g/24 hours
9-12 Years 0.20-1.40 g/24 hours
13-17 Years 0.40-1.90 g/24 hours
>17 Years 0.50-2.15 g/24 hours

Clinical Significance
Urinary Free Cortisol is useful in the detection of patients with Cushing's syndrome for whom Free Cortisol concentrations are elevated.

Performing Laboratory
Quest Diagnostics Nichols Institute San Juan Capistrano
33608 Ortega Hwy
San Juan Capistrano, CA 92675

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.