Vitamin D, 25-Hydroxy Total[LC/MS/MS]

Test Code
VITD TOTAL (92888)

Quest Code
92888

CPT Codes
82306

Includes
Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2

Preferred Specimen
0.5 mL serum

Patient Preparation
Fasting preferred, but not required

Minimum Volume
0.3 mL

Instructions
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a serum separator tube, allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from serum separator tube gel or clot within 48 hours • Grossly icteric

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Sun-Sat

Report Available
1 to 2 days

Reference Range
Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established


Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.

Performing Laboratory
Quest Diagnostics Nichols Institute Valencia
27027 Tourney Road
Valencia, CA 91355-5386



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.