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Immunofixation, 24 Hour Urine Protein
MessagePerforming Lab: Central Lab
Test Code
3624
Alias/See Also
Sunquest: IFE24; Immunoelectrophoresis, Urine Bence Jones Protein
CPT Codes
86335
Preferred Specimen
20 mL of a 24-hour urine collection
Minimum Volume
10.0 mL
Instructions
Collection:
Specimen must be from a 24 hour urine collection
Processing: Do not add preservatives to the specimen
Specimen must be from a 24 hour urine collection
Processing: Do not add preservatives to the specimen
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Preservative present in sample
Methodology
Agarose Gel Electrophoresis with Immunoprecipitation
Setup Schedule
Tuesday and Thursday
Report Available
Within 5 days
Reference Range
No Free Light Chains or Monoclonal Protein Detected
Clinical Significance
Immunoglobulin free light chains as well as heavy chain fragments may be seen in the urine of patients with conditions such as multiple myeloma, macroglobulinemia, primary systemic amyloidosis or light chain deposition disease. Immunofixation is the process used to identify the immunoglobulin heavy chain and/or light chain.
