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Vitamin D,25-Hydroxy, Total
MessagePerforming Lab: Central Lab, Sartell
Test Code
4583
Alias/See Also
Sunquest: VTD25, Vitamin D 25-OH
CPT Codes
82306
Preferred Specimen
0.2 mL Lithium Heparin Plasma (light green-top) tube
Minimum Volume
0.1 mL
Other Acceptable Specimens
Serum (gold-top) tube
Instructions
Sartell ONLY
Order on separate accession number. May be combined with IPTH only.
Order on separate accession number. May be combined with IPTH only.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature (3 days), Refrigerated (12 days), Frozen (3 months)
Methodology
Chemiluminescent microparticle immunoassay (CMIA)
Setup Schedule
Daily
Report Available
Same Day
Reference Range
30-80 ng/mL
Clinical Significance
25-OH Vitamin D is the metabolite that is the most reliable clinical indicator of Vitamin D status. Vitamin D is a fat soluble steroid hormone that comes in two forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D is synthesized from cholesterol upon skin exposure to UVB sunlight and through dietary intake. Vitamin D deficiency may be linked to chronic diseases such as cancer (prostate, breast, colon) as well as cardiovascular disease, osteoporosis, osteomalacia, and several autoimmune diseases. Diagnosis of Vitamin D Deficiency and related conditions such as Osteomalacia, Osteoporosis, Rickets as well as the diagnosis of Vitamin D overdose. Measurement of Vitamin D has become increasingly important in the management of patient with various disorders of calcium metabolism associated with neonatal hypocalcaemia, pregnancy, and post-menopausal osteoporosis.
