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Heparin, Unfractionated
MessagePerforming Lab: Regions, Hudson, Westfields, Lakeview
Test Code
3719
Alias/See Also
Sunquest: HEPUF; Beef or Porcine Heparin
CPT Codes
85520
Preferred Specimen
2.7 mL (in 3 mL tube) 3.2% Sodium Citrate Plasma (light blue-top) tube filled to fill line on the tube
Minimum Volume
1.0 mL plasma aliquot per test
Other Acceptable Specimens
1.0 mL platelet poor plasma (frozen)
Instructions
Processing: Freeze an aliquot of platelet poor plasma at -20C if not performed within 4 hours of collection
Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Transport Container
Transport processed samples frozen on Dry Ice
Transport Temperature
Frozen if > 4 hours old
Specimen Stability
Room Temperature: 4 hours Refrigerated: 4 hours Frozen 72 hours at -20C or 2 weeks at -80C
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Refrigerated or Room temperature >4 hours
Hemolyzed or Clotted Samples, Samples do not meet the minimum draw requirements, Samples exceed maximum draw requirements, Samples with hematocrit >55% not collected with the special tube. Samples from patients on Direct Xa inhibitors,
Hemolyzed or Clotted Samples, Samples do not meet the minimum draw requirements, Samples exceed maximum draw requirements, Samples with hematocrit >55% not collected with the special tube. Samples from patients on Direct Xa inhibitors,
Methodology
Chromogenic
Setup Schedule
Daily
Report Available
Same Day
Reference Range
0.30-0.70 U/mL
Critical Value: >=1.0 U/mL
Critical Value: >=1.0 U/mL
Clinical Significance
The quantitative determination of plasma Unfractionated Heparin and Low Molecular Weight Heparin levels are useful for monitoring treatment efficacy of thromboembolic diseases.
