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Hepatitis B DNA PCR Quantitative
MessagePerforming Lab: Central Lab
Test Code
4685
Alias/See Also
Sunquest: HBVQT; Hepatitis B DNA real-time PCR, Quantitative (Viral Load)
CPT Codes
87517
Preferred Specimen
Serum Separator FULL (tall gold-top) tube
Minimum Volume
1.5 mL
Other Acceptable Specimens
Red, Red/Gray
Instructions
Collection:
Centrifuge within 6 hours of collection. Send the parent tube to Central Lab, no need to send aliquot.
Processing:
Remove or separate serum within 6 hours of collection.
Shipping:
Decant the serum into a separate “PCR Acceptable” transport vial.
Must use SPECIAL PCR transport vials with screw-caps. To avoid cross-contamination, please handle each patient specimen and decant PCR tube with a separate set of clean gloves.
Centrifuge within 6 hours of collection. Send the parent tube to Central Lab, no need to send aliquot.
Processing:
Remove or separate serum within 6 hours of collection.
Shipping:
Decant the serum into a separate “PCR Acceptable” transport vial.
Must use SPECIAL PCR transport vials with screw-caps. To avoid cross-contamination, please handle each patient specimen and decant PCR tube with a separate set of clean gloves.
Transport Container
Send original/parent collection vial
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 7 days once serum is separated from cells
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Any plasma or non-serum specimen
Methodology
Real Time Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Monday - Friday (HBV Qt Run at least twice per week)
Report Available
Within 5 days of collection
Limitations
Based on Practice Guidelines recommended by the American Association for the Study of Liver
Disease (AASLD), The HBV PCR Quant test may be ordered (along with Hepatitis Be Ag & Hepatitis BeAb) as an additional reflex test for patients found to be Positive (Reactive) for Hepatitis B surface Antigen (HBsAg). Refer to the separate catalog pages for Hepatitis B surface antigen for clarification on which HBsAg order codes to use. The AASLD guidelines can be accessed on the web at the following address: http://www.aasld.org/practiceguidelines
HBV PCR Quant specimens resulted as Detected are reported to the Department of Health.
Disease (AASLD), The HBV PCR Quant test may be ordered (along with Hepatitis Be Ag & Hepatitis BeAb) as an additional reflex test for patients found to be Positive (Reactive) for Hepatitis B surface Antigen (HBsAg). Refer to the separate catalog pages for Hepatitis B surface antigen for clarification on which HBsAg order codes to use. The AASLD guidelines can be accessed on the web at the following address: http://www.aasld.org/practiceguidelines
HBV PCR Quant specimens resulted as Detected are reported to the Department of Health.
Reference Range
Not Detected
Clinical Significance
This HBV Quantitative assay is a Real-Time Polymerase Chain Reaction (RT-PCR) assay for the quantitation of the Hepatitis B virus. It tests over a linear range of 10 – 1,000,000,000 IU/ml (1.00 – 9.0 log IU/ml) and is approved to report detectable levels below 10 IU / ml. This assay is FDA-approved to detect HBV Genotypes A – H. It is intended for use in the conjunction with clinical presentation and other laboratory markers for disease prognosis and for use as an aid in assessing viral response to antiretroviral treatment as measured by changes in serum HBV DNA levels. Based on Practice Guidelines recommended by the American Association for the Study of Liver Disease (AASLD), The HBV PCR Quant test may be ordered (along with Hepatitis Be Ag & Hepatitis BeAb) as an additional reflex test for patients found to be Positive (Reactive) for Hepatitis B surface Antigen (HBsAg). Refer to the separate catalog pages for Hepatitis B surface antigen for clarification on which HBsAg order codes to use. The AASLD guidelines can be accessed on the web at the following address: http://www.aasld.org/practiceguidelines
