Bilirubin, Direct

Message

Performing Lab: Central Lab, Hudson, Lakeview, Regions, Sartell, Westfields

Test Code

0105

Alias/See Also

Sunquest: BILD; Direct Bilirubin, Conjugated Bilirubin

CPT Codes
82248

Preferred Specimen

100 uL Lithium Heparin Plasma (light green-top) tube

Minimum Volume

10 uL

Other Acceptable Specimens

Serum (gold-top, red-top, red/gray-top), Lithium Heparin (light green-top) on ICE

Instructions

Collection:
Protect from light. Exposure to light may falsely decrease result.

Transport Temperature

Refrigerated

Specimen Stability

Room Temperature: 2 days
Refrigerated: 7 days
Frozen: 3 months at -20 C

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross Hemolysis

Methodology
Diazo Reaction

Setup Schedule

Hospital: Daily
Clinics: Monday - Friday

Report Available

Same day.

Limitations

Bilirubin is photosensitive and samples should be protected from both sunlight and fluorescent light to avoid photo-degradation.

Reference Range

Adult: 0.0-0.5 ml/dL

Clinical Significance

Jaundice has been classified as unconjugated and conjugated hyperbilirubinemia. Increased plasma-unconjugated bilirubin is commonly seen in hemolytic disorders, Gilbert’s syndrome, Crigler-Najjar syndrome, neonatal jaundice, and ineffective erythropoiesis and in the presence of drugs competing for glucuronide. Increased plasma-conjugated bilirubin occurs with hepatobiliary disorders, including intrahepatic and extrahepatic biliary tree obstruction, liver cell damage, Dubin-Johnson syndrome, and Rotor syndrome.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.