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Kleihauer Betke
MessagePerforming Lab: Regions
Test Code
4624
Alias/See Also
Sunquest: KBT; Fetal Cell Stain; Fetal Maternal Bleed, Fetal Maternal Hemorrhage
CPT Codes
85460
Includes
Fetal bleed results of >75 mL will be reviewed by a pathologist
Preferred Specimen
1.0 mL EDTA Whole Blood (pink-top) tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
EDTA (lavender-top)
Instructions
HP Clinics: Usually needed STAT. Always send to Regions via special courier. Ensure ABRH (ABO/Rh testing) is ordered and sent to Regions Blood Bank.
Testing takes approximately 4 hours after receipt of specimen at Regions
Testing takes approximately 4 hours after receipt of specimen at Regions
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Citrate or Heparin samples. Cord Blood
Methodology
Microscopic examination
Setup Schedule
Daily
Report Available
Same Day
Limitations
HbF may occur in relatively high concentrations in adult patients with inherited disorders of
erythropoiesis such as maternal sickle cell anemia and thalassemia, and persistence of fetal hemoglobin.
erythropoiesis such as maternal sickle cell anemia and thalassemia, and persistence of fetal hemoglobin.
Reference Range
Normal values for HbF in adults are below 0.01%; in full term newborns they are above 90%, therefore, a negative result should have essentially no fetal cells present
Clinical Significance
The determination of the level of fetal red blood cells in maternal circulation is important in the management of women with a suspected uterine trauma. This test is used to determine if a fetal-maternal hemorrhage (FMH) has occurred
