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Prenatal Quad Screen
MessagePerforming Lab: Central Lab
Test Code
3777
Alias/See Also
Sunquest: PQUAD
CPT Codes
84702, 82105, 82677, 86336
Preferred Specimen
0.5 mL Serum Separator (gold-top) tube
Minimum Volume
0.4 mL
Other Acceptable Specimens
Red, Red/Gray
Instructions
Ordering:
If testing for ONTD risk only, order AFP, Maternal Serum (EPIC code 3363)
Collection:
Samples must be collected between 15 – 20 completed weeks gestation. The optimal time to collect the sample is between 16 – 18 weeks gestation.
Processing:
Freeze at -20º C if not tested within 48 Hours of collection.
If testing for ONTD risk only, order AFP, Maternal Serum (EPIC code 3363)
Collection:
Samples must be collected between 15 – 20 completed weeks gestation. The optimal time to collect the sample is between 16 – 18 weeks gestation.
Processing:
Freeze at -20º C if not tested within 48 Hours of collection.
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 48 hours
Frozen: 1 month
Frozen: 1 month
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly hemolyzed, lipemic, or icteric specimens.
Specimen not frozen within 48 Hours of collection
Specimen not frozen within 48 Hours of collection
Methodology
Chemiluminescence
Setup Schedule
Tuesday, Wednesday, Friday
Report Available
Same day
Limitations
Risks for Down Syndrome and Trisomy 18 will not be calculated on pregnancies involving multiple fetuses
For assays employing antibodies, the possibility exists for interference by heterophile antibodies in the patient sample. Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies, e.g. HAMA, that interfere with immunoassays. Additionally, other heterophile antibodies such as human anti-goat antibodies may be present in patient samples. Such interfering antibodies may cause erroneous results. Carefully evaluate the results of patients suspected of having these antibodies.
For assays employing antibodies, the possibility exists for interference by heterophile antibodies in the patient sample. Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies, e.g. HAMA, that interfere with immunoassays. Additionally, other heterophile antibodies such as human anti-goat antibodies may be present in patient samples. Such interfering antibodies may cause erroneous results. Carefully evaluate the results of patients suspected of having these antibodies.
Reference Range
No Elevated Risk for ONTD, Down Syndrome and Trisomy 18
Risks reported are Second Trimester Risks
The Cutoff for ONTD risk is = 2.5 MoM
The Cutoff for Down Syndrome is 1:270
The Cutoff for Trisomy 18 is 1:100
Risks reported are Second Trimester Risks
The Cutoff for ONTD risk is = 2.5 MoM
The Cutoff for Down Syndrome is 1:270
The Cutoff for Trisomy 18 is 1:100
Clinical Significance
The Quad Screen is a screening test used to help identify pregnancies at increased risk for open neural tube defects (spina bifida/anencephaly), Down syndrome (Trisomy 21) or Trisomy 18.
