Galactose-1-Phosphate, Erythrocytes [34510X]

Test Code
5282

CPT Codes
84378<br />

Preferred Specimen

Red cell pellet from heparinized whole blood submitted in a plastic aliquot, leak-proof plastic vial (see instructions)



Patient Preparation
**Samples for this test CAN NOT be collected at Patient Service Centers (PSC)**

Instructions
Erythrocytes must be washed within 4 hours of draw.
Collection container/Tube: Green top (heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: Washed, packed cells (red cell pellet from step "F" in collection instructions).

Collection:
1.) Collect and process a minimum of 2 mL of whole blood as follows:
A. Immediately centrifuge for 10 minutes at 650 x g. For conversion to RPMs, see Additional Information.
B. Discard the plasma and buffy coat layers.
C. Add a cold 0.9% saline solution to the erythrocytes (about 2 times the volume of erythrocytes). D. Mix gently by inversion and centrifuge again for 10 minutes at 650 x g.
E. Remove and discard the saline.
F. Repeat the wash steps (C-E) 2 more times.
2.) After the final centrifugation, remove and discard the saline and a thin layer of the top cells.

Additional information: The relative centrifugal force (G-force) can be estimated by applying the following formula: g=11.18 x r (n/1000)2. Where: r=radius in centimeters and n=speed in RPM. The radius from the center of the rotation axis to the bottom or outermost portion of the test tube should be used. RCF is expressed relative to the force of the earth's gravity.
Forms: If not ordering electronically, submit a Biochemical Genetics Request Form (Supply T439) with the specimen.

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 9 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Anticoagulants other than heparin

Methodology
Ultraviolet • Enzymatic

Setup Schedule
Set up: Wed; Report available: 10 days

Reference Range

Non-galactosemic: 5-49 mcg/g of hemoglobin (<1 mg/dL)
Galactosemic on galactose restricted diet: 80-125 mcg/g of hemoglobin (1-4 mg/dL)
Galactosemic on unrestricted diet: >125 mcg/g of hemoglobin (>4 mg/dL)



Performing Laboratory
Mayo Medical Laboratories
200 First Street SW
Rochester, MN 55905



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.