Sarcoma Mutation Analysis Panel, Pediatric [19884X]

Test Code
19884

CPT Codes
81401 (x5), 81479

Preferred Specimen
Minimum: 0.35 cm3 formalin-fixed, paraffin-embedded, tissue block

Other Acceptable Specimens
30 mg fresh, flash-frozen tissue submitted in a sterile, plastic leak-proof container

Transport Temperature
Formalin fixed paraffin embedded tissue: Room temperature
Fresh unfixed flash frozen tissue: Frozen

Specimen Stability

Formalin fixed paraffin embedded tissue:
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable

Fresh unfixed tissue:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 1 year (-70°)



Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status

This test was developed and its performance characteristics have been determined by Quest Diagnostics. Performance characteristics refer to the analytical performance of the test.



Setup Schedule
Set up: Mon a.m.; Report available: 5 days

Reference Range

Not detected



Clinical Significance
This panel test is used to identify the translocations listed below:
EWS-WT1 translocation t(11;22) (q13;q12)
EWS-FLI1 translocation t(11;22) (q24;q12)
EWS-ERG translocation t(21;22) (q22;q12)
PAX3/FKHR translocation t(2;13) (q35;q14)
PAX7/FKHR translocation t(1;13) (p36;q14)
SYT-SSX1 translocation t(X;18) (p11.23;q11.2)
SYT-SSX2 translocation t(X;18) (p11.21;q11.2)


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.