Factor X Activity, Clotting [359X]

Test Code
29203

CPT Codes
85260

Preferred Specimen
1 mL frozen platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
0.5 mL

Instructions
Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid WBS/platelet buffy layer, and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice.

General Drawing Instructions:
Refer to Quest Diagnostics Nichols Institute Directory of Services under Specimen Collection section, Coagulation Testing, for further information on specimen processing.

Transport Container
Plastic screw-cap vial

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen -20° C: 14 days
Frozen -70° C: 1 year

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis

Methodology
Photometric Clot Detection

Setup Schedule
Set up: Mon-Fri evening; Report available: Next day

Limitations
Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: decreased, Heparin (UFH or LMWH): no effect (reagent contains heparin neutralizer); Dabigatran or Argatroban (Thrombin Inhibitors): no effect to inhibitor pattern or falsely low; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect to inhibitor pattern.

Reference Range
70-150 % normal

Clinical Significance
Bleeding disorders may be due to inherited or acquired
deficiencies of a clotting factor.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.