Bartonella DNA, Real-Time PCR [11108X]

Test Code
6639

CPT Codes
87471<br /> **This test is not available for New York patient testing**

Includes
Bartonella henselae, Bartonella quintana

Preferred Specimen
0.7 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume
Whole blood: 0.3 mL
Tissue: 3 (1 mm)

Other Acceptable Specimens
Whole blood collected in: ACD-B (yellow-top) tube • 3 (3 mm) tissue, submitted in a sterile, plastic, leak-proof container

Transport Temperature
Whole blood (preferred): Refrigerated (cold packs)
Tissue: Frozen

Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
Tissue
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Mon-Sat a.m.; Report available: 2-3 days

Limitations
B. quintana and B. henselae as well as chlamydia species and Coxiella burnetii may cross-react and produce false-positive results.

Reference Range
B. henselae DNA, Qual. PCR Not detected
B. quintana DNA, Qual. PCR Not detected


Clinical Significance
Bartonella DNA PCR is a highly specific and sensitive method to detect the presence of Bartonella species DNA in clinical specimens. This assay can differentiate between Bartonella henselae and Bartonella quintana. The diagnosis of Bartonella infection should not rely solely on the result of a PCR assay. A negative PCR result indicates only the absence of Bartonella species DNA in the sample tested and does not exclude the diagnosis of the disease. Patients with a positive PCR result should be evaluated with other tests to further establish the diagnosis of the disease. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests.

Performing Laboratory
Quest Diagnostics Infectious Disease, Inc.
33608 Ortega Highway, Bldg B-West Wing
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.