Culture, Ureaplasma urealyticum

Test Code
5499

CPT Codes
87109

Preferred Specimen
Minimum: 1 mL or 1 swab of urogenital specimen (vaginal, cervical, urethral swabs or vaginal secretions) collected in VCM medium (green-top), or M4 Tranpsort Media, or UTM

Other Acceptable Specimens
Urine, sterile body fluids, tissue, wounds (swab), respiratory (sputum, bronchial washing, tracheobronchial secretions, bronchial alveolar lavage, nasopharyngeal or throat swabs) collected in a VCM medium (green-cap) tube or equivalent (UTM) container (see instructions)

Instructions
Ship on dry ice.
Urine:
Client should centrifuge urine at 3000 rpm for 15 minutes. Suspend sediment in VCM or equivalent transport media.

If the specimen is not centrifuged, submit a 1:1 volume of urine in VCM or equivalent transport media.

Submit a 1:1 volume of sterile body fluids, tissue, respiratory (sputum, bronchial washing, tracheobronchial secretions, bronchial alveolar lavage) in VCM or equivalent transport media.

Respiratory samples are only acceptable from children under 1 year old.

Please note: Do not use M4RT; the room temperature formula cannot be used for mycoplasma.

Transport Container
VCM medium (green-top), or M4 Tranpsort Media, or UTM

Transport Temperature
Frozen -70°C

Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen -30° C: Unacceptable
Frozen -70° C: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens collected on wooden shafted swabs, or cotton swabs • Specimens received in expired transport medium • Tissue specimens in formalin • Urine containing any preservatives • Specimens received in M4RT transport medium • Raw specimens • Specimen collected in molecular transport medium

Methodology
Culture

Setup Schedule
Set up: Daily a.m., p.m., and (night shifts); Report available: 10 days

Reference Range
Not isolated

Clinical Significance
Ureaplasma urealyticum and Mycoplasma hominis are primarily associated with genital tract colonization and disease in adults and respiratory tract colonization and disease in newborns. Though controversial, these organisms have been associated with endometritis, chorioamnionitis, premature rupture of membranes, stillbirth, premature birth, low birth weight, post-partum infections, and infertility. Of particular concern is the causal relationship between central nervous system infections in the premature newborn and U. urealyticum.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.