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| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Mycoplasma hominis Culture
Test Code3888
CPT Codes
87109
Preferred Specimen
Minimum: 1 mL or 1 swab of urogenital specimen (vaginal, cervical, urethral swabs or vaginal secretions) collected in VCM medium (green-top), or M4 Tranpsort Media, or UTM
Other Acceptable Specimens
Urine, sterile body fluids, tissue, wounds (swab), respiratory (sputum, bronchial washing, tracheobronchial secretions, bronchial alveolar lavage, nasopharyngeal or throat swabs) - see instructions
Instructions
Ship on dry ice.
Urine: Client should centrifuge urine at 3000 rpm for 15 minutes. Suspend sediment in VCM or equivalent transport media. If the specimen is not centrifuged, submit a 1:1 volume of urine in VCM or equivalent transport media.
Submit a 1:1 volume of sterile body fluids, tissue, respiratory (sputum, bronchial washing, tracheobronchial secretions, bronchial alveolar lavage) in VCM or equivalent transport media.
Respiratory samples are only acceptable from children under 1 year old.
Please note: Do not use M4RT; the room temperature formula cannot be used for mycoplasma.
Urine: Client should centrifuge urine at 3000 rpm for 15 minutes. Suspend sediment in VCM or equivalent transport media. If the specimen is not centrifuged, submit a 1:1 volume of urine in VCM or equivalent transport media.
Submit a 1:1 volume of sterile body fluids, tissue, respiratory (sputum, bronchial washing, tracheobronchial secretions, bronchial alveolar lavage) in VCM or equivalent transport media.
Respiratory samples are only acceptable from children under 1 year old.
Please note: Do not use M4RT; the room temperature formula cannot be used for mycoplasma.
Transport Container
VCM Transport Medium, or M4 Transport Media, or UTM
Transport Temperature
Frozen -70°C
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Refrigerated: 48 hours
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens collected on wooden shafted swabs, or cotton swabs • Specimens received in expired transport medium • Tissue specimens in formalin • Urine containing any preservatives • Specimens received in M4RT transport medium • Raw specimens • Specimen collected in molecular transport medium
Methodology
Culture
Setup Schedule
Set up: Daily; Report available: 10 days
Reference Range
Not isolated
Clinical Significance
Ureaplasma urealyticum and Mycoplasma hominis are primarily associated with genital tract colonization and disease in adults and respiratory tract colonization and disease in newborns. Though controversial, these organisms have been associated with endometritis, charioamnionitis, premature rupture of membranes, stillbirth, premature birth, low birth weight, post-partum infections, and infertility. Of particular concern is the casual relationship between central nervous system infections in the premature newborn and U. urealyticum.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
