Streptococcus Group B DNA, PCR with Broth Enrichment

Test Code

CPT Codes
87081, 87150

Preferred Specimen
Vaginal/Rectal swab in Amies gel (blue-cap) transport medium or Amies liquid (red-cap) transport medium

Other Acceptable Specimens
Vaginal/Rectal swab in Liquid Stuart transport medium or 2 mL LIM broth tube (pre-incubated)

In order to obtain an adequate specimen, the procedure for specimen collection must be followed closely. Using recommended swab, vaginal-rectal specimens are collected according to the following procedure:

1. Wipe away excessive amount of secretion or discharge from the vaginal area.
2. Carefully insert the swab into the lower one-third part of vagina, and sample secretions from the mucosa.
3. Carefully insert the same swab, approximately 2.5 cm beyond the anal sphincter, and gently rotate to sample anal crypts.
4. Replace the swab in its container.

If submitting broth, inoculate LIM broth with vaginal/rectal swab and incubate at 35-37° C for 18-24 hours.

Note: Endocervical, perianal, perineal and perirectal are not to be used for this test, and a speculum should not be used for culture collection.

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Amies transport medium
Room temperature: 24 hours
Refrigerated: 6 days
Frozen: Unacceptable

LIM broth tube
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Specimens in transport media other than those listed • LIM broth received room temperature

Culture Enhanced • Polymerase Chain Reaction (PCR)

Setup Schedule
Set up: Daily; Report available: 3-4 days

Reference Range
Not detected

Clinical Significance
Detect Group B Streptococcus (GBS) vaginal/rectal colonization to prevent GBS sepsis and other GBS infections in newborns.

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.